Ultrastructure, Solubilization and Protein Composition of Eggshell (Chorion) of Gesonula punctifrons (Stal, 1861) (Orthoptera: Acrididae)

Authors

Keywords:

Gesonula punctifons, eggshell, SEM, TEM, SDS-PAGE.

Abstract

Developmental changes in ultrastructure and polypeptide composition of chorion of Gesonula punctifrons (Orthoptera: Acrididae) have been studied by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Sodium Dodecyl Sulfate-Poly Acrylamide Gel Electrophoresis (SDS-PAGE). Along with morphogenesis and maturation of chorion pentagonal and hexagonal architecture and ‘cap’ like structure appeared in the surface of the eggshell. Transmission Electron Microscopic studies revealed presence of protein fiber deposition and their intercalation with polysaccharides. SDS-PAGE studies showed presence of five polypeptide bands in all the developmental stages and suggested possibilities of aggregate formation among different polypeptide components.

Author Biographies

  • Arpita SHYAM ROY, Tripura University
    PhD Scholar, Department of Zoology, Tripura University
  • Durgadas GHOSH, Tripura University
    Professor of Zoology, Department of Zoology, Tripura University

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Published

03.12.2014

Issue

Vol. 16 No. 3 (2014): Journal of the Entomological Research Society

Section

Journal of the Entomological Research Society

How to Cite

Ultrastructure, Solubilization and Protein Composition of Eggshell (Chorion) of Gesonula punctifrons (Stal, 1861) (Orthoptera: Acrididae). (2014). Journal of the Entomological Research Society, 16(3), 45-54. https://entomol.org/journal/index.php/JERS/article/view/661