Physiological and Biochemical Effects of Klebsiella oxytoca Infection on Model Organism Galleria mellonella L

Abstract

Examination of physiological and biochemical effects on infection models is very important for the application of new treatments against human pathogens. It is used as an infection model because the innate immune responses of insects are similar to those of mammals. In this study, the physiological response of model insect Galleria mellonella to human pathogenic bacteria Klebsiella oxytoca infection was investigated. Changes in metabolic enzyme activities such as aspartate transferase (AST), alanine transferase (ALT), alkaline phosphatase (ALP), amylase (AMYL) and gamma glutamyl transferase (GGT) occurred due to K. oxytoca infection. In addition, amounts of non-enzymatic antioxidant such as uric acid (UA), bilirubin (BIL) and albumin (ALB) were altered in G. mellonella hemolymph due to infection. The results obtained from this study showed that G. mellonella can be used as a model organism in the evaluation of the physiological effects of K. oxytoca infection on the host.